Expression and clinical significance of microRNA-148a in patients with non-small cell lung cancer
10.3760/cma.j.issn.1673-422X.2016.08.003
- VernacularTitle:微小RNA-148a在非小细胞肺癌中的表达及其临床意义
- Author:
Yukai PAN
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small-cell lung;
MicroRNAs;
Pathological conditions,signs and symptoms;
Survival analysis
- From:
Journal of International Oncology
2016;43(8):570-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of microRNA-148a (miR-148a) in non-small cell lung cancer (NSCLC) tissues and adjacent non-tumor tissues and the relationships with clinicopathologic features,to analyze the roles of miR-148a in evaluating prognosis.Methods Expression levels of miR-148a in 48 pairs of NSCLC and adjacent non-tumor tissues were detected by quantitative real-time PCR (RT-PCR).The patients were divided into 2 groups according to the median value of miR-148a.The relationships between the expression levels of miR-148a and clinicopathological features were analyzed.The survival rate was estimated by Kaplan-Meier method,and single factor analysis was performed.The relationship between the expression level of miR-148a and prognosis was analyzed by Log-rank test.Results miR-148a expression levels were significant down-regulated in NSCLC tissues compared with adjacent non-tumor tissues (1.052 ±0.659 vs.1.397 ± 0.667,t =2.549,P =0.013).miR-148a expression level was correlated with tumor size (x2 =4.594,P =0.030),lymph nodes metastasis (x2 =5.486,P =0.019) and clinical stage (x2 =4.090,P =0.043),while it was not correlated with age (x2 =0.354,P =0.551),gender (x2 =2.277,P =0.131),histological type (x2 =0.403,P =0.525) and smoking (x2 =0.444,P =0.505).Kaplan-Meier analysis revealed a significant correlation between low miR-148a expression level and poor overall survival (23.9 months vs.38.7 months,x2 =4.941,P =0.026),and no significant correlation with progression-free survival (21.6 months vs.29.4 months,x2 =2.168,P =0.141).Conclusion Down-regulation of miR-148a is correlated with worse clinicopathological features,which serves an independent marker for poor prognosis in patients with NSCLC.
