Isoflurane inhibits proliferation and differentiation of neural stem cells in the dentate gyrus
10.3969/j.issn.2095-4344.2015.19.017
- VernacularTitle:异氟醚麻醉抑制海马齿状回神经干细胞的增殖及分化
- Author:
Chengkang LU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(19):3049-3053
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Isoflurane is gradualy popularized in pediatric surgery due to its rapid onset, rapid recovery, and no savings, but its security needs to be further studied. OBJECTIVE:To explore the effect of isoflurane anesthesia on the proliferation and neuronal differentiation of neural stem cels in the dentate gyrus of neonatal rats. METHODS: Rats were randomly divided into isoflurane group and control group, respectively treated with isoflurane inhalation and air inhalation. 5-Bromodeoxyuridine bal glycosides (BrdU) was intraperitonealy injected before drug administration and after drug withdrawal, and 24 hours after secondary injection, the rats were executed to take brain tissues for detection of BrdU expression and brain neurogenic differentiation factor expression. RESULTS AND CONCLUSION:After cessation of anaesthesia processing for the detection of blood glucose and arterial blood gas, a mild rise in PaCO2 was found in the isoflurane group and pH showed a slight drop, but PaO2, BE, SaO2 and blood glucose levels were not changed. Compared with the control group, the isoflurane group did not appear with obvious hypoxia performances after isoflurane anesthesia, including cyanosis and respiratory depression. Rat neural stem cels of the dentate gyrus were mostly distributed in the gate area, and compared with the control group, the number of BrdU-positive cels was less in the isoflurane group (P < 0.05). A large number of new cels in the dentate gyrus expressed NeuroD, and the number of NeuroD+/BrdU+ cels in the gate area was significantly higher than that in the lower region of granule cels. Moreover, the number of NeuroD+/BrdU+ cels in the isoflurane group was significantly higher than that in the control group (P < 0.05). These findings suggest that isoflurane anesthesia can influence the proliferation and neuronal differentiation of neural stem cels in the dentate gyrus of neonatal rats, which inhibits cel proliferation and promotes neuronal differentiation.
