In vitro effects of apolipoprotein A5 on macrophage : implication in arthrosclerosis
10.3760/cma.j.issn.1000-6699.2012.08.015
- VernacularTitle:载脂蛋白A5体外对吞噬细胞的作用及抗动脉硬化机制
- Publication Type:Journal Article
- Keywords:
apolipoprotein A5;
Adenovirus;
Inflammatory cytokines;
Foam cells;
Atherosclerosis
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(8):666-668
- CountryChina
- Language:Chinese
-
Abstract:
The PAW-264.7 macrophages,transfected with apolipoprotein A5 ( apoA5 ) adenovirus or LacZ adenovirus,were challenged with liposome (LPS) or acetylated low-density lipoprotein (acLDL).Production of representatiwe inflammatory cytokine was examined by measuring cytokines in the culture medium with ELISA.Foam cells were counted under a fluorescent microscope. Incorporation of H3-labelled cholesterol was used to reflect cholesterol transportation by the macrophages. The cholesterol and triglyceride were measured with a RIA method.apoA5 transfection decreased the secretion of inflammatory cytokine,the formation of foam cells,as well as the cholesterol transportation.In conclusion,apoA5 could attenuate atherosclerosis by inhibiting the production of inflammatory cytokines and the formation of foam cells,and may represent a potential target for the prevention and treatment of atherosclerosis.
