The effect of temperature on the neutrophil activation in the cardiopulmonary bypass
10.3760/cma.j.issn.1001-4497.2011.010.011
- VernacularTitle:不同体外循环温度下离体中性粒细胞的激活
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass;
Temperature;
Membrane-bound elastase;
In vitro
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2011;27(10):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective Neutrophils,as important participants in the inflammatory response,contribute virtually to the tissue and organ damage in the cardiopulmonary bypass (CPB) We tried to investigate the contributing factors and mechanism for neutrophil activation in CPB at various temperature in vitro.Methods Neutrophils isolated from the blood of 60 healthy volunteers were divided into 5 temperature groups normothermia,tepid temperature,moderate hypothermia,deep hypothermia and rewarming hyperthermia groups.An in vitro model was established with a polymerase chain reaction thermocycler for evaluating neutrophil activation Six time points were set in each group:T0 standing for baseline,T1 for starting rewarming,T2 for 0.5,T3 for 1,T4:for 1.5 and T5 for 2 hours after rewarming.Membrane-bound elastase (MBE) activity,as an index for neutrophil activation,was measured every 0.5 hours throughout the experiment.SPSS 13.0 was used for intra-group and intergroup comparisons.Analysis of variance ( repeat measure ANOVA ) was used for comparisons among different time points.Bonferroni method would be used for pairwise comparison if a significant difference was observed between two effects with analysis of variance.Results For inter-group comparisons of MBE at different time points:at T1,T2 and T3 time points,the activity of MBE in the normothermia group was higher than that in the tepid temperature group and far more higher than in the other groups ( P ＜ 0.05 ).There was no significant difference in the activity of MBE between normothermia and tepid temperature groups but the values of MBE in the two groups were higher than those in the other groups at T4 and T5 time points ( P ＜ 0.05 ).The comparisons among rewarming hyperthermia group,moderate hypothermia group and deep hypothermia group varied with time points:at T1,T2 and T5 time points,there were no significant difference( P ＞ 0.05 ) ; however,at T3 time point,the activity of MBE in the rewarming hyperthermia group was higher than that in the moderate hypothermia and deep hypothermia groups (P＜0.05) ; at T4 time point,the activity of MBE in the moderate hypothermia group and rewarming hyperthermia group was higher than that in the deep hypothermia group( P ＜0.05 ).Conclusion Neutrophil activation changed dynamically with the temperature in CPB.Normothermia and tepid temperature promoted while moderate hypothermia and deep hypothermia inhibited the activation Recovery for neutrophil activation was faster in the rewarming hyperthermia than in the moderate hypothermia,and the recovery in profound hypothermia was the slowest after a hypothermic inhibition.
