Effects of emodin on proliferation, apoptosis and expression of apoptotic regulating gene of human pancreatic cancer cell line BxPC3
- VernacularTitle:大黄素对人胰腺癌细胞BxPC3增殖及凋亡基因表达的影响
- Publication Type:Journal Article
- Keywords: Pancreatic neoplasms; Emodin; Apoptosis; Cell cycle; Apoptotic regulating gene
- From: Chinese Journal of Pancreatology 2008;8(5):298-301
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of emodin on cell growth, proliferation cycle and its effect on the expression of apoptotic regulating gene of human pancreatic cancer cell line Bxpc-3 in vitro. Methods The survival rates of BxPC3 cell in vitro under the treatment of different concentrations emodin (0, 10, 20, 40, 80μmol/L) were detected by MTT colorimetric assay; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis; the expression of Bax, Bak, Bad and Bid mRNA was assessed by reverse transcriptase polymerase chain reaction (RT-PCR). Results (1) The inhibitory effort of emodin on BxPC3 cell proliferation was found to be in a dose-dependent manner along with the concentration of emodin changed from 0 μmol/L to 80 μmol/L, the cell survival rates were (97.42±2.45)%, (78.58± 3.11) %, (62.39±2.19) %, (51.68±2.92) %, (34.30±4.04)%; (2) Cell cycle changes were present in the groups treated with emodin, the percentage of cells in G0/G1 stage increased in a dose-dependent manner along with the concentration of emodin changed from 0 μmol/L to 80 μmol/L, the percentages of cells in G0/G1stage were (51.22±0.64)%, (53.88±0.72)%, (55.39±1.12)%, (58.17±1.48)%, (63.72± 1.52)%, and that in S stage decreased (42.87±0.67)%, (39.68±0.58)%, (34.60±1.06)%, (31.88± 1.48) %, (27.26±1.67)%; (3) The apoptotic rates of the groups treated with emodin raised in a dose-dependent manner, along with the concentration of emodin changed from 0 μmol/L to 80 μmoL/L, the cell apoptotic rates were (19.16±1.69)%, (31.78±2.21)%, (47.03±3.39)%, (55.92±5.39)%, (62.78± 3.19)%;(4)The expression of bax, bak, bad and Bid mRNA in BxPC3 cell treated with emodin was up-regulated significantly (F=55.649, P<0.01; F=19.403, P<0.05;F=29.009, P<0.05;F=39.546, P < 0.01). Conclusions Emodin was able to inhibit proliferation and induce the apoptosis of pancreatic cancer cell line BxPC3 effectively in vitro, and these effects were possibly associated with the up-regulation of bax, bak, bad and bid mRNA expression.