Analysis of HPV-other Samples by Performing HPV DNA Sequencing.
- Author:
Yoo Duk CHOI
1
;
Chang Woo HAN
;
Woon Jae CHUNG
;
Woon Won JUNG
;
Ji Shin LEE
;
Jong Hee NAM
;
Min Cheol LEE
;
Sang Woo JUHNG
;
Ho Sun CHOI
;
Chang Soo PARK
Author Information
1. Department of Pathology, Chonnam National University Medical School, Gwangju, Korea. daniel5438@hanmail.net
- Publication Type:Original Article
- Keywords:
DNA chips;
Papillomaviridae;
Sequence analysis, Uterine cervix
- MeSH:
Biopsy;
Cervix Uteri;
Chimera;
DNA;
Female;
Humans;
Oligonucleotide Array Sequence Analysis;
Papillomaviridae;
Sequence Analysis, DNA
- From:Korean Journal of Pathology
2009;43(3):250-253
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing. METHODS: For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high-risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV-other samples of 209 patients who subsequently underwent cervical biopsy. RESULTS: For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types. Conclusion: HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.
