Low Dose Treatment of RhTGF-beta1 Restore the Wound Healing Defect in Dexamethasone-treated Rats.
10.11637/kjpa.2010.23.4.207
- Author:
Ki Young KANG
1
;
Hyun KIM
Author Information
1. Department of Anatomy, College of Medicine, Seonam University, Korea.
- Publication Type:Original Article
- Keywords:
rhTGF-beta1;
TGF-beta;
Wound healing;
Dexamethasone
- MeSH:
Animals;
Collagen;
Dexamethasone;
Fibroblasts;
Humans;
Intercellular Signaling Peptides and Proteins;
Macrophages;
Monocytes;
Rats;
Rats, Sprague-Dawley;
Re-Epithelialization;
Transforming Growth Factor beta;
Transforming Growth Factor beta1;
Wound Healing
- From:Korean Journal of Physical Anthropology
2010;23(4):207-215
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Transforming growth factor-beta1 (TGF-beta1) has chemotactic effect to monocytes, macrophages and fibroblasts, and it stimulates those cells to produce growth factors and collagen fibers. Therefore, TGF-beta1 plays important roles in the wound healing process. The aim of this study is to evaluate the effects of recombinant human TGF-beta1 (rhTGF-beta1) on the wound healing defect in dexamethasone-treated rats. Dexamethasone (0.1 mg/kg/day) was subcutaneously injected into the eight-week-old Sprague-Dawley rats. At the third day of dexamethasone administration, full thickness wounds were made on the back of the rat. And then, rhTGF-beta1 (50 ng/wound) was intradermally injected in the vicinity of the wounds. The wounds of the normal and the negative control groups were provided with vehicle only. At 1, 3, 5, 7 and 15 days after the surgery, the wound sizes were measured and the wound tissues were obtained and analyzed. The mean wound sizes of the rhTGF-beta1 injected groups were significantly smaller than those of the negative control groups. Moreover, the histologic findings such as cellular infiltration, re-epithelialization and granulation formation and expression patterns of TGF-betas during the wound healing process were improved by administration of rhTGF-beta1. These results support that rhTGF-beta1 can restore wound healing defect due to dexamethasone administration.
