Analysis of microbial diversity of supra-gingival plaque in dental caries
10.3760/cma.j.issn.1002-0098.2014.12.010
- VernacularTitle:龋病患者龈上菌斑微生物多样性的研究
- Author:
Weiyue WU
1
;
Benxiang HOU
Author Information
1. 100050,首都医科大学口腔医学院牙体牙髓科
- Keywords:
Dental plaque;
16SrDNA;
Polymerase chain reaction-denaturing gradient gel electrophoresis;
Cloning and sequencing
- From:
Chinese Journal of Stomatology
2014;49(12):742-747
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the microbial composition and differences in dental plaque of caries and caries-free adults.Methods Caries-active(n=16) was defined as an individual who had at least three untreated decayed tooth and decayed-missing-filled-tooth(DMFT) score greater than 6.Caries-free (n=16) was defined as an individual who had a DMFT score equal to zero.The patients were 18-35 years old.Samples from supra-gingival plaque were obtained and PCR-denaturing gel electrophoresis cloning and sequencing of caries pathogens were used to catch the core microbial of dental caries.Results Six phylum,28 genus and 88 species were detected.In caries group,Prevotella,Capnocytophaga,Actinomyces,Veillonella and Corynebacterium were predominant,accounting for 56.2％ (334/594) of the total cloning number of caries group.Caries-free group contained more predonminant genus than caries group.Prevotella,Veillonella,Capnocytophaga,Corynebacterium,Streptococcus,Actinomyces,Aggregatibacter and Neisseria were predominant,accounting for 65.2％ (354/543) of the total cloning number of caries-free group.Caries group had less diversity than the caries-free group.The difference was statistically significant(P＜0.05).Conclusions Caries might be caused by potentially pathogenic microbial communities rather than a single pathogen.In the progress of dental caries,the microbial diversity decreased.
