Effect of advanced glycation end products on the human gingival fibroblast proliferation and typeⅠcollagen synthesis
10.3321/j.issn:1002-0098.2008.01.005
- VernacularTitle:糖基化终产物对牙龈成纤维细胞增殖和Ⅰ型胶原合成的影响
- Author:
Liu-Ying QI
1
;
Yun FU
;
Yan ZHOU
Author Information
1. 中山大学光华口腔医学院
- Keywords:
Glycosylation end products,advanced;
Fibroblasts;
Collagen;
Cell proliferation
- From:
Chinese Journal of Stomatology
2008;43(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply the synthesized advanced glycation end products(AGE)to the cultured human gingival fibroblast(HGF)in vitro and then to investigate the effects of AGE on the HGF proliferation and type Ⅰ collagen synthesis and the potential impact of AGE in the repair of periodontium and its molecular mechanism in diabetes-associated periodontitis. Methods The HGF was obtained from explants of human healthy gingival tissues by using tissue-explant technique.The AGE Was prepared and then added to the culture media, its effect on HGF proliferation at different time duration Was examined with MTT colorimetric assay.The type Ⅰ collagen concentrations in cell culture supematants and intracellular proteins were detected by EUSA,and the type Ⅰ collagen mRNA expression of HGF was analyzed by realtime RT-PCR. Results 200 mg/L AGE decreased the A value(P ＜0.05)and changed the HGF shape.Incubation of HGF with AGE for 72 hours,the quantities of type Ⅰ collagen were reduced(P＜0.05),and the expression of type Ⅰ collagen mRNA Was down-regulated(P＜0.05). Condusions The AGE inhibited the HGF proliferation,decreased the synthesis of type Ⅰ collagen and down-regulated the expression of type Ⅰ collagen mRNA,impairing the repair of periodontium.
