Detection of epidermal growth factor receptor gene mutations and its clinical significance in non-small cell lung cancers.
- Author:
Jing ZHAO
1
;
Yan ZHU
;
Li ZHANG
;
Yong-wei YU
;
Jian-ming ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; Adenocarcinoma, Bronchiolo-Alveolar; genetics; Adult; Aged; Carcinoma, Non-Small-Cell Lung; genetics; pathology; Exons; Female; Genes, erbB-1; Humans; Lung Neoplasms; genetics; pathology; Male; Middle Aged; Mutation; Mutation Rate; Polymerase Chain Reaction; methods; Receptor, Epidermal Growth Factor; genetics; Sequence Analysis, DNA; methods; Sex Factors; Smoking
- From: Chinese Journal of Pathology 2011;40(10):671-674
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the detection technology and its clinical significance of the EGFR gene mutation in non-small cell lung cancer.
METHODSDNA direct sequencing methods by PCR amplification were used to detect EGFR gene exons 18-21 mutation and to analyze its clinical pathological significance in 192 patients with non-small cell lung cancer.
RESULTS64 of the 192 cases presented with EGFR gene tyrosine kinase binding domain mutation (64/192, 33.3%), of which exon 19 deletion mutation rate was 60.9% (39/64), exon 21 alternative mutation rate was 39.1% (25/64), but exons 18 and 20 mutation was not found in this group of patients. EGFR gene mutation rate was 58.5%(24/41) in lung adenocarcinoma associated with bronchioloalveolar carcinoma differentiation, which was significantly higher than that of ordinary adenocarcinoma (37.9%, 33/87), squamous cell carcinoma (7.5%, 4/53), large cell carcinoma (1/5) and adenosquamous carcinoma (2/6, P<0.05). EGFR gene mutation rates in male patients (20.9%, 24/115), were significantly higher than in the females (51.9%, 40/77; P<0.01); non-smokers (50.0%, 57/114), significantly higher than that of smokers (9.0%, 7/78; P<0.01).
CONCLUSIONSDNA direct sequencing method by PCR amplification is stable and reliable in detection of EGFR gene mutation in non-small cell lung cancer. It might provide a scientific basis for targeted therapy.
