Expression and clinical significance of miR-21 in diffuse large B cell lymphoma.
- Author:
Chun-Hong LI
1
;
Rong FU
2
;
Yi-Hao WANG
1
;
Wen-Jing SONG
1
;
Er-Bao RUAN
1
;
Wen QU
1
;
Hua-Quan WANG
1
;
Guo-Jin WANG
1
;
Jia SONG
1
;
Xiao-Ming WANG
1
;
Yu-Hong WU
1
;
Li-Min XING
1
;
Hong LIU
1
;
Li-Juan LI
1
;
Jing GUAN
1
;
Zong-Hong SHAO
3
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Female; Humans; Lymphoma, Large B-Cell, Diffuse; metabolism; pathology; Male; MicroRNAs; metabolism; Middle Aged; PTEN Phosphohydrolase; metabolism; Real-Time Polymerase Chain Reaction
- From: Journal of Experimental Hematology 2014;22(2):339-343
- CountryChina
- Language:Chinese
- Abstract: This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma (DLBCL) paraffin-embedded tissues, and evaluate its potential relevance with clinical characteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymph node tissue specimens were examined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining. The results indicated that the expression of miR-21 was significantly higher in tumor tissues [6.586(1.10,38.22)] than that in normal tissues [0.791 (0.35,2.87)] (P < 0.05). Among 26 patients with DLBCL the expression of PTEN protein was positive in 6 patients (23%), and was negative in 20 patients (77%). In patients with DLBCL, the expression level of miR-21 was negatively correlated with the level of PTEN protein. The high expression of miR-21 was positively correlated with the level of serum LDH. The expression level of miR-21 in patients with Ann Arbor III-IV stage was obviously higher than that of patients with Ann Arbor I-II stage, but did not correlate with the subtype of patients in clinic (P > 0.05). It is concluded that the expression of miR-21 is high in DLBCL and its overexpression may be related with poor prognosis of DLCBL. These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL.
