Effects of rhG-CSF mobilization on the polarization and migration of donor's CD4(+)T lymphocytes.
10.7534/j.issn.1009-2137.2014.02.037
- Author:
Zhen-Yang GU
1
;
Meng LI
1
;
Sha-Sha ZHAO
1
;
Bo CAI
1
;
Xiao-Li ZHAO
1
;
Li-Xun GUAN
1
;
Li-Li WANG
1
;
Chun-Ji GAO
2
Author Information
1. Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
2. Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China. E-mail: gaochunji@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
CD4-Positive T-Lymphocytes;
cytology;
drug effects;
Case-Control Studies;
Cell Movement;
Female;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Humans;
Male;
Middle Aged;
Recombinant Proteins;
pharmacology;
Tissue Donors;
Young Adult
- From:
Journal of Experimental Hematology
2014;22(2):470-474
- CountryChina
- Language:Chinese
-
Abstract:
The polarization and migration of T lymphocytes involves the adhesive interaction of lymphocyte function-associated antigen 1(LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) mobilization on the polarization and migration of donor's CD4(+) T cells in peripheral blood. The peripheral blood mononuclear cells were collected from 10 healthy volunteers and 10 donors on the fifth day of mobilization with rhG-CSF. And the CD4(+)T cells were purified by miniMACS. The polarization and migration of CD4(+) T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by using inverted and confocal microscopes respectively. The results showed that the percentage of polarized CD4(+)T cells from donors(32.42 ± 4.91)% was lower than that from healthy controls(56.55 ± 5.35)% (P < 0.01), the migration velocity of CD4(+)T cells from donors (7.06 ± 1.44 µm/min) was also lower than that of healthy controls(9.05 ± 1.91 µm/min)(P < 0.01). It is concluded that the polarization and migration of CD4(+)T lymphocytes is impaired after rhG-CSF mobilization.
