Detection of luxS gene of quorum sensing signal system in periodontal pathogen

VernacularTitle:牙周致病菌密度感应信号系统luxS基因的检测
Author: Choo-Feng LEI ¹ ; He YANG ; Chang-Juan SUN ; Di MIAO ; Yi XU
Author Information

1. 四川大学华西口腔医学院
Keywords: Periodontics; Oligonucleotide array sequence analysis; Quorum sensing
From: Chinese Journal of Stomatology 2009;44(1):32-34
CountryChina
Language:Chinese
Abstract: Objective To detect the presence and distribution of luxS gene in quorum sensing signal system in the periodontal pathogens.Methods The total DNA of Porphyromonas gingivalis(Pg),Fusobacterium nucleatum(Fn),Actinobacillus acitinomycetimcomtans(Aa)were extracted.The presence of luxS was detected by polymerase chain reaction(PCR).The products of PCR were detected by electrophoresis.sequenced and identified by a Blast search of the GenBank database. Results Electrophoresis,sequencing and Blast searching indicated that the PCR products of Pg were highly consistent with the luxS gene in GenBank.The sequencing result of Fn was also identified with the target gene.The PCR product of Aa was the same as reference through electrophoresis.Conclusions Pg,Fn,Aa contain luxS gene.Further studies may be required to investigate the functions of luxS in the periodontal pathogens.