Immunofluorescence examination of CK-13 expression tn cell line KB differentiated by all-trans retinoic acid or As2O3

VernacularTitle:免疫荧光法检测全反式维甲酸和As2O3对人口腔鳞状细胞癌KB细胞的诱导分化作用
Author: Xiao-Hong ZHAO ¹ ; Hua-Jun ZHU ; Da-Peng LU ; Min ZHANG
Author Information

1. 首都医科大学口腔医学院
Keywords: Keratin-13:Tretinoin: Arsenic trioxide
From: Chinese Journal of Stomatology 2009;44(1):53-55
CountryChina
Language:Chinese
Abstract: Objective To examine the expression of cytokeratin-13(CK-13)in oral squamous cell carcinoma(OSCC)and to discuss the effects of all-trans retinoic acid(ATRA)or arsenic trioxide(As2O3)on the differentiation of human oral undifferentiated squamous cell carcinoma cell line KB cells.Methods The cultured KB cells were divided into three groups,ATRA group,As2O3 group,and control The expression of CK-13 in KB cells was detected using the inmmunofluorescence before and after KB cells were induced by ATRA or As2O3.Results The expression rates of CK-13 in KB cells in the ATRA group and As2O3 group were significantly higher than that in the control(P＜0.05),but there was no significant difference in the expression between ATBA and As2O3 group(P＞0.05).Conclusions ATRA and As2O3 both have the ability to differentiate the KB cells,and the expression is associated with the degree of tumor differentiation.CK-13 may serve as a molecular marker to evaluate the effect of the differentiation treatment on OSCC.