A differential expression proteomic study of human periodontal ligament cell during osteogenic differentiation
10.3760/cma.j.issn.1002-0098.2009.04.005
- VernacularTitle:人牙周韧带细胞成骨分化的差异表达蛋白质组学研究
- Author:
Li-Ping WU
1
;
Xi WEI
;
JUN-Qi LING
;
Lu LIU
Author Information
1. 中山大学光华口腔医学院
- Keywords:
Proteomics;
Electrophoresis,gel,two-dimensional;
Human periodontal ligament cell;
Osteogenic differentiation
- From:
Chinese Journal of Stomatology
2009;44(4):212-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain regulating proteins during human periodontal ligament cell (hPDLC) nstengenic differentiation and investigate its underlying molecular mechanism. Methods Two-dimension difference in gel electrophoresis (2D-DIGE) coupled with matrix-assissted laser adsorption ionization-time of flight mass spectrometry ( MALDI-TOF-MS ) analysis was used to identify regulating proteins during hPDLC osteogenic differentiation. Results A differently expressed protein profile was obtained 7 days after osteogenic induction, and 61 protein spots with significant differences, 29 protein spots were identified by MALDI-TOF-MS, including cytoskeleton, cell membrane-bounding, nuclear regulation,matrix synthesis, and metabolic enzymes and signal tnmsduction and other proteins. A functional class of cyteskeleton proteins showed co-regulation, these proteins were intimately involved in the reorganization of the cytoskeleton, cytokinesis and migration during PDLC differentiation. Conclusions A database of differently displayed proteins during hPDLC osteogenic differentiation was established, which may be helpful to understand and further study molecular mechanism of PDLC osteogenic differentiation.
