Expression of hnRNP A2/B1 in human lung cancer cell lines.

Author: Chi WU ¹ ; Weimin LI ; Wenbin CHEN ; Dan XU ; Dapeng WEI ; Qinghua ZHOU
Author Information

1. Division of Respiratory, Department of Internal Medicine, Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Publication Type:Journal Article
From: Chinese Journal of Lung Cancer 2004;7(2):121-124
CountryChina
Language:Chinese
Abstract:
BACKGROUNDTo explore the mRNA expression of heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 in human lung cancer cell lines.
METHODSExpression of hnRNP A2/B1 and hnRNP B1 mRNA was detected by RT-PCR using specific primers. Expression of hnRNP B1 protein was examined by immunocytochemistry using specific anti-hnRNP B1 antibody.
RESULTSRT-PCR indicated that the hnRNP A2/B2 and hnRNP B1 mRNA existed in the lung adenocarcinoma cell lines SPC-A1 and Y-90, squamous cell carcinoma cell line A431 and small cell lung cancer cell line NCI-H446. According to immunocytochemistry results, the expression of hnRNP B1 was observed in lung cancer cell lines, but the expression in squamous cell carcinoma cell line A431 and small cell lung cancer cell line NCI-H446 was markedly higher than that in adenocarcinoma cell lines SPC-A1 and Y-90.
CONCLUSIONSThe results suggest that the human lung cancer cell lines detected have overexpression of hnRNP A2/B1 and hnRNP B1.