Interaction among Rb/pl6,Rb/E2F1 and HDAC1 Proteins in Gallbladder Carcinoma
10.1007/s11596-009-0611-5
- Author:
WANG XIN
1
;
HUANG KAI
;
XU LINING
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Rb;
pl6;
E2F1;
HDAC1;
gallbladder carcinoma;
cell line;
protein interaction
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(6):729-731
- CountryChina
- Language:Chinese
-
Abstract:
The mechanism and interaction among Rb/p16,Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method,the interactions among Rb,pl6,E2F1,HDAC1 proteins in gallbladder carcinoma cell line (Mz-ChA-1) were studied.It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1,and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb,HDAC1 and E2F1 proteins in gallbladder carcinoma,indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb,HDAC1,and E2F1 proteins.
