Expression of TRAF-6,TAK1 and TGF-β mRNA in peripheral blood mononuclear cells of patients with diffuse large B cell lymphoma before and after chemotherapy.
10.7534/j.issn.1009-2137.2014.05.022
- Author:
Zhen-Zhu CHEN
1
;
Qing-Song YIN
2
,
3
;
Xu-Dong WEI
1
;
Lin CHEN
1
;
Yu-Fu LI
1
Author Information
1. Department of Hematology, The Affiliated Cancer Hospital of Zhengzhou University (Heman Province Cancer Hospital), Zhengzhou 450008, Henan Province, China.
2. Department of Hematology, The Affiliated Cancer Hospital of Zhengzhou University (Heman Province Cancer Hospital), Zhengzhou 450008, Henan Province, China. E-mail: jnyinqingsong@
3. com.
- Publication Type:Journal Article
- MeSH:
Gene Expression Regulation, Leukemic;
drug effects;
Humans;
Leukocytes, Mononuclear;
drug effects;
metabolism;
Lymphoma, Large B-Cell, Diffuse;
drug therapy;
genetics;
MAP Kinase Kinase Kinases;
genetics;
RNA, Messenger;
genetics;
Signal Transduction;
TNF Receptor-Associated Factor 6;
genetics;
Transforming Growth Factor beta;
genetics
- From:
Journal of Experimental Hematology
2014;22(5):1301-1305
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to detect the expression levels of TRAF6, TAK1 and TGF-β mRNA in peripheral blood mononuclear cell (PBMNC) of patients with diffuse large B cell lymphoma (DLBCL) before and after chemotherapy, and to explore the effect of chemotherapy on the activity of TRAF6/TAK1 signal pathway. The expression levels of TRAF-6, TAK1 and TGF-β mRNA in PBMNC of 38 patients with DLBCL were detected by using the quantitative real time PCR before treatment or after two cycles of chemotherapy, 12 healthy people were served as the control. The results showed that the expression levels of TRAF-6, TAK1 and TGF-β mRNA in PBMNC of DLBCL patients' were higher than those in healthy people. Before treatment, the expression levels of TRAF-6 and TAK1 mRNA had no significant difference as compared with healthy people (P > 0.05); after chemotherapy, the expression levels of these two genes significantly increased, and the differences both had statistically significant as compared with healthy people (P < 0.05); meanwhile the increased expression levels of these two genes after chemotherapy had statistically significant difference as compared with levels before treatment (P < 0.05) , and those expression levels were positively correlated. While the expression level of TGF-β mRNA decreased after chemotherapy as compared with level before treatment, and the differences had statistically significantse(P < 0.05). It is concluded that the activity of TRF6/TAK1 signal pathways in PBMNC of DLBCL patients' significantly increases after chemotherapy, while the expression level of TGF-β mRNA after chemotherapy is abviously lower than level before treatment.
