Preliminary study on genotypic diversity of Streptococcus mutans in Xinjiang Uygur preschool children
10.3760/cma.j.issn.1002-0098.2011.05.003
- VernacularTitle:新疆维吾尔族儿童乳牙龋与变形链球菌基因型相关性的初步研究
- Author:
Jing LIN
1
;
Jin ZHAO
;
Nurbiye MUNAMMETEMIN
;
Xin-Shang LI
Author Information
1. 新疆医科大学第一附属医院
- Keywords:
Uygur nationality;
Streptococcus mutans;
Genotype;
Arbitrarily primed polymerase chsin reaction
- From:
Chinese Journal of Stomatology
2011;46(5):267-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between genotypic diversity of Streptococcus mutans(Sm)and deciduous teeth caries in Xinjiang Uygur autonomous region.Methods Isolates of Sm were obtained from 17 caries-free and 17 caries-active Uygur preschool children aged from 3 to 5 years.A total of 143 isolates were subcultured,biochemically characterised and identified by polymerase chain reaction(PCR)as Sm and Streptococcus sobrinus(Ss).Then the Sm isolates were genotyped by arbitrarily primed-PCR(AP-PCR).Results A total of 81 Sm isolates from caries-active subjects and 62 isolates from caries-free subjects were identified by PCR and forty distinct genotypes identified from 143 clinicalisolates.In seventeen caries-active group,9 children had only 1 genotype,5 children had 2 genotypes,3 children had 3 genotypes.In seventeen caries-free group,14 children had only 1 genotype,3 children had more than 2 genotypes.The Spearman correlation test showed a strong associatiou between genotypic diversity and caries experience(r=0.342.P<0.05).Conclusions Isolates of Sm in caries-active Uygur preschool children show apparent more genetic diversity than those in caries-free children.The genotypes of isolates might be related to differences in caries susceptibility.
