Comparative study on specific chromatograms and main nucleosides of cultivated and wild Cordyceps sinensis.
10.19540/j.cnki.cjcmm.20170807.009
- Author:
Ke ZAN
1
;
Li-Li HUANG
1
;
Li-Nong GUO
1
;
Jie LIU
1
;
Jian ZHENG
1
;
Shuang-Cheng MA
1
;
Zheng-Ming QIAN
1
;
Wen-Jia LI
1
Author Information
1. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Cordyceps sinensis;
HPLC;
cultivated;
nucleosides;
specific chromatograms;
wild
- From:
China Journal of Chinese Materia Medica
2017;42(20):3957-3962
- CountryChina
- Language:Chinese
-
Abstract:
This study is to establish the HPLC specific chromatogram and determine four main nucleosides of wild and cultivated Cordyceps sinensis. Uridine, inosine, guanosine and adenosine were selected as reference substance. HPLC analysis was performed on a Waters XSelect HSS T3 C₁₈ (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water(A)-acetonitrile (B) at a flow rate of 0.6 mL•min⁻¹ (0-5 min,0% B;5-15 min,0%-10% B, 15-30 min,10%-20% B, 30-33 min, 20%-50% B, 33-35 min, 50%-0% B, 35-40 min, 0% B). The detection wavelength was 260 nm and the column temperature was controlled at 30 ℃, and the injection volume was 5 μL. HPLC specific chromatogram of wild and cultivated C. sinensis was established and four main nucleosides were simultaneously determined by the above method. Specific chromatograms and contents of four main nucleosides showed no significant differences between cultivated and wild C. sinensis. These results can provide scientific evidences for further development and utilization of cultivated C. sinensis.
