Identification of saponins from Panax notoginseng in metabolites of rats.
10.19540/j.cnki.cjcmm.20170901.006
- Author:
Wen-Wen SHEN
1
;
Yin ZHANG
1
;
Shou-Bei QIU
1
;
Fen-Xia ZHU
1
;
Xiao-Bin JIA
1
;
Dao-Quan TANG
2
;
Bin CHEN
1
Author Information
1. The Third Clinical Medical College of Nanjing University of Chinese Medicine (Affiliated Provincial Hospital of Integrated Traditional Chinese Medicine and Western Medicine), Key Laboratory for Traditional Chinese Medicine Drug Release System under State Administration of Traditional Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
2. Jiangsu Provincial Key Laboratory for New Drug Research and Clinical Pharmacy of Xuzhou Medical University, Xuzhou 221004, China.
- Publication Type:Journal Article
- Keywords:
UPLC-QTOF-MS/MS;
ginsenoside Rh₄, Rk₃, Rk₁, Rg₅;
identification of metabolites;
n-butanol extract derived from Panax notoginseng;
transformation mode
- From:
China Journal of Chinese Materia Medica
2017;42(20):3996-4001
- CountryChina
- Language:Chinese
-
Abstract:
UPLC-QTOF-MS/MS was used to identify metabolites in rat blood, urine and feces after the administration of n-butanol extract derived from steamed notoginseng. The metabolic process of saponins came from steamed notoginseng was analyzed. The metabolites were processed by PeakView software, and identified according to the structural characteristics of prototype compounds and the accurate qualitative and quantitative changes of common metabolic pathways. Four saponins metabolites were identified based on MS/MS information of metabolites, namely ginsenoside Rh₄, Rk₃, Rk₁, Rg₅,and their 15 metabolites were verified. The metabolic pathways of the four ginsenosides in n-butanol extract included glucuronidation, desugar, sulfation, dehydromethylation, and branch loss. The metabolites of main active saponin components derived from steamed Panax notoginseng were analyzed from the perspective of qualitative analysis. And the material basis for the efficacy of steamed notoginseng was further clarified.
