Effect of BRD4 Inhibitor GSK525762A on Proliferation and Apoptosis of SUP-B15 Cells In Vitro and Its Possible Mechanism.

Xue WANG; Na QI; Sha MA; Zhi-Ling YAN; Qing-Yun WU; Lin WANG; Chong CHEN; Kai-Lin XU

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Effect of BRD4 Inhibitor GSK525762A on Proliferation and Apoptosis of SUP-B15 Cells In Vitro and Its Possible Mechanism.

Author: Xue WANG ¹ ; Na QI ¹ ; Sha MA ² ; Zhi-Ling YAN ² ; Qing-Yun WU ¹ ; Lin WANG ¹ ; Chong CHEN ³ ; Kai-Lin XU ^{4
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Author Information

1. Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
2. Department of Hematology, The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, Jiangsu Province, China.
3. Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail:chenchong@xzmc.edu.cn.
4. Department of Hematology, The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, Jiangsu Province, China. E-mail: lihmd@
5. com.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(6):1654-1658
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of BRD4 inhibitor GSK525762A on the proliferation and apoptosis of Philadelphia chromosome-positive acute lymphoblastic leukemia SUP-B15 cells and its mechanism.
METHODSSUP-B15 cells were treated with different concentration of GSK525762A, the proliferation-inhibition curve was assayed and plotted by CCK-8 method, the cell viability and apoptosis were detected by flow cytometry with Annexin V and 7-AAD staining. The transcripts of anti-apoptotic genes C-MYC, CDK6 and BCL-2 were detected by real-time PCR.
RESULTSGSK525762A could inhibit significantly SUP-B15 cell proliteration in dose-and time-dependent manner; GSK525762A treatment could induce apoptosis of SUP-B15 cells. The levels of C-MYC,CDK6 and BCL-2 mRNA transcripts in SUP-B15 cells were reduced in GSK525762A-treated group.
CONCLUSIONThe GSK525762A can remarkably inhibit proliferation and induce apoptosis of SUP-B15 cells. The down-regulation of apoptosis-related genes C-MYC,CDK6 and BCL-2 may be involved in the process of apoptosis.