Cytocompatibility of nanophase hydroxyapatite ceramics.
- Author:
Bo WEN
1
;
Zhi-qing CHEN
;
Yin-shan JIANG
;
Zheng-wen YANG
;
Yong-zhong XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biocompatible Materials; Cell Adhesion; Cell Proliferation; Cells, Cultured; Ceramics; Durapatite; Nanostructures; Osteoblasts; cytology; Rats
- From: West China Journal of Stomatology 2004;22(6):456-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the cytocompatibility of nanophase hydroxyapatite ceramics in vitro.
METHODSHydroxyapatite (HA) was prepared via wet method. The grain size of the hydroxyapatite in the study was determined by scanning electron microscope and atomic force microscope with image analysis software. Primary osteoblast culture was established from rat calvaria. Cell adherence and proliferation on nanophase hydroxyapatite ceramics and conventional hydroxyapatite ceramics were examined at 1, 3, 5, 7 days. Morphology of the cells was observed by microscope.
RESULTSThe average grain size of the nanophase and conventional HA was 55 nm and 780 nm, respectively. Throughout 7 days period, osteoblast proliferation on the HA was similar to that on tissue culture borosilicate glass controls, osteoblasts could attach, spread and proliferate on HA. However, compared to conventional ceramics, osteoblast proliferation on nanophase HA was significantly better after 1, 3, 5 and 7 days.
CONCLUSIONCytocompatibility of nanophase HA was significantly better than conventional ceramics.
