Effects of Heparin-Binding Epidermal Growth Factor on the Expression of MMP-9 and ATPase gamma-subunit mRNA in the Mouse Embryo.
- Author:
Seok Ho HONG
1
;
Hee Young NAH
;
Ji Yun LEE
;
Jung Hee KIM
;
Chung Hoon KIM
;
Hee Dong CHAE
;
Byung Moon KANG
;
Moon Kyoo KIM
Author Information
1. Department of Biology, College of Natural Sciences, Hanyang University, Korea.
- Publication Type:Original Article
- Keywords:
ATPase g-subunit;
Blastocoel;
HB-EGF;
MMP-9;
RT-PCR
- MeSH:
Adenosine Triphosphatases*;
Animals;
Blastocyst;
Embryonic Structures*;
Epidermal Growth Factor*;
Matrix Metalloproteinase 9;
Mice*;
RNA, Messenger*
- From:Korean Journal of Fertility and Sterility
2001;28(2):87-94
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase g-subunit mRNA. METHODS: Late 2-cell mouse embryos was cultured for 72 hours in HTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase gamma-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase g-subunit was higher than other groups. CONCLUSIONS: Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos.
