OBJECTIVETo explore the effect of Shengxue Zengbai Decoction on the cell cycle and apoptosis in hematopoietic rebuilding in mice after bone marrow transplantation (BMT).

METHODSThe experimental animals were randomly divided into 5 groups: the normal group (with no treatment), the model group, the granulocyte-colony stimulating factor (G-CSF) group, the Shengxue group, and the Shengxue + G-CSF group. BMT mouse model was established. Shengxue Zengbai Decoction concentrated decoction was given by gastrogavage to mice in the Shengxue group and the Shengxue + G-CSF group. On Day 9, 14, and 24 after BMT, the apoptotic rates of myelomonocytes were detected using flow cytometry. Changes of the cell cycle were analyzed. Peripheral blood cells and myelomonocyte were counted. Histological observation was performed.

RESULTSOn Day 14 after BMT the level of hemoglobin in the peripheral blood cells was higher in the Shengxue group than in the model group and the G-CSF group (P < 0.05). The level of hemoglobin in the Shengxue +G-CSF group was higher than that in the model group (P < 0.05). On Day 24 after BMT the level of white blood cells in the peripheral blood cells in the Shengxue group was higher than that in the model group (P < 0.05). On Day 24 after BMT the myelomonocyte count in the G-CSF group, the Shengxue group, and the Shengxue + G-CSF group was higher than in the model group (P < 0. 01). The cell apoptotic rate on Day 24 after BMT was lower in the Shengxue group than in the G-CSF group and the model group of the same period.

CONCLUSIONShengxue Zengbai Decoction could promote the proliferation and differentiation of bone marrow hematopoietic cells, inhibit the apoptosis of bone marrow cells, which might be one of the mechanisms for hematopoietic rebuilding of Shengxue Zengbai Decoction.