Clopidogrel metabolism related gene polymorphisms in Chinese patients with acute coronary syndrome
10.3760/cma.j.issn.0253-3758.2012.11.003
- VernacularTitle:急性冠状动脉综合征患者氯吡格雷代谢基因多态性分析
- Author:
Guang-Xun FENG
1
;
Yan LIANG
;
Ying BAI
;
Tao CHEN
;
Xin LIU
;
Yan-Min YANG
;
Xing-Yu WANG
;
Yue-Jin YANG
;
Jun ZHU
Author Information
1. 100037,中国医学科学院北京协和医学院阜外心血管病医院急重症中心
- Keywords:
Coronary disease;
Platelet aggregation inhibitors;
Polymorphisms,single nucleotide
- From:
Chinese Journal of Cardiology
2012;40(11):908-913
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the single nucleotide polymorphisms of clopidogrel metabolism related genes (CYP2C19,ABCB1 and PON1) in Chinese patients with acute coronary syndrome(ACS) by genotype analysis.Methods Genetic analysis was performed in patients admitted to Fuwai Hospital from 2005 to 2008 with ACS within 4 weeks.The detection of polymorphisms was performed by TaqMan real-time PCR method.The alleles genotyped were CYP2C19 * 2-* 8,* 17,ABCB1 C3435T,PON1 Q192R and PON1 L55M.Minor allele frequency (MAF) was calculated.Patients were classified as one of the 5 categories by clopidogrel metabolizer pheuotypes as extensive [without any "loss-of-function" (LOF) allele * 2-* 8 or "gain-of-function" (GOF) allele * 17],intermediate (with only one LOF allele),Poor (with two or more LOF alleles),ultra (with one or two GOF alleles) or unknown (with one LOF allele and one GOF allele).Results A total of 2800 ACS patients were enrolled [mean age (59.0 ± 12.3) years and 2236 males(79.9％)].There were 74％ patients with ST-segment elevation myocardial infarction (STEMI,n =2072),22.0％ patients with non-ST-segment elevation myocardial infarction (NSTEMI,n =617)and 4.0％ patients with unstable angina (UA,n =111).The minor allele frequency (MAF) for each genotype of CYP2C19 *2,*3,*4,*17 was 28.7％,4.6％,0.1％ and 1.2％,respectively.There was noLOF allele * 5-* 8 in the study population.The MAF for ABCB1 C3435T,PON1 Q192R and PON1 L55M was 39.4％,37.8％ and 4.4％,respectively.Clopidogrel metabolizer groups were defined as extensive in 41.7％,intermediate in 45.6％,poor in 10.3％,ultra in 1.9％ and unknown in 0.6％ patients,respectively.There were no significant differences for all genotypes between males and females.Total LOF carriers of CYP2C19 were 56.4％ and GOF carriers were 2.5％.Conclusions Our study demonstrated a high distribution of the LOF allele of CYP2C19 in China ACS population.
