Expression of PDCD5 and caspase 3 in the cochlea of different age of C57BL/6J mice
10.3760/cma.j.issn.1673-0860.2011.09.011
- VernacularTitle:PDCD5和caspase3在不同年龄段C57BL/6J小鼠耳蜗中的表达
- Author:
Yan WANG
1
;
Han-Qi CHU
;
Liang-Qiang ZHOU
;
Jin CHEN
;
Zhi-Yong LI
;
Yun LIU
;
Ping ZHANG
;
Xiao-Wen HUANG
;
Yong-Hua CUI
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Presbycusis;
Apoptosis;
Neoplasm proteins;
Apoptosis regulatory proteins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(9):747-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the age related changes of the expression of programmed cell death 5 (PDCDS) and caspase 3 in the cochlea of the different age of C57 BL/6J mice. The relationship of PDCD5 and caspase 3 and the possible roles in the pathogenesis of presbycusis were also discussed.Methods C57 mice of 3, 6, 9 and 12 months old were selected and divided into 4 groups, with 15 mice in each group. Auditory function of C57BL/6J mice was measured by auditory brainstem response (ABR) respectively. The changes of PDCD5 and Caspase 3 protein in the cochlea were detected by immunohistochemistry and Western blotting, the changes of PDCD5 mRNA and caspase 3 mRNA were detected using RT-PCR. Results With the increase of age, the mean value for ABR thresholds in response to click, 4 kHz and 8 kHz sound stimulus of C57 mice gradually increased,the expression of PDCD5 and caspase 3 were increased also. At 3 months and 6 months of age in the cochlea of C57, all sorts of expression of PDCD5 and caspase 3 and the expression were enhanced with age. There was an evident expression at 9 months age, but the highest expression was detected at 12 months age. The PDCD5 and Caspase 3 expression were statistically different in each group ( P ＜ 0. 05 ). The changes of PDCD5 and caspase 3 mRNA expression were in accordance with that of PDCD5 and Caspase 3 protein expression by the realtime PCR. Conclusions The expression levels of PDCD5 and caspase 3 in the cochlea of C57 mice increase with age, the results suggested that the expression of PDCD5 and caspase 3 might play an important role in the pathogenic mechanism of presbycusis.
