Functional reconstruction with tissue engineered myoblast in facial muscle of rat.
- Author:
Guilin HUANG
1
;
Longjiang LI
;
Yuming WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cells, Cultured; Collagen Type I; pharmacology; Facial Muscles; injuries; pathology; surgery; Female; Gels; Male; Myoblasts; cytology; transplantation; Rats; Rats, Sprague-Dawley; Reconstructive Surgical Procedures; Tissue Engineering
- From: West China Journal of Stomatology 2003;21(6):432-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.
METHODSUsing purified, subcultured myoblast of neonatal rats and type I collagen gels as extracellular matrix (ECM) and scaffold, tissue engineered muscle was transplanted in the face of syngeneic nutured rats.
RESULTSTissue engineered myoblast generated and differentiated in vitro were observed with microscope. Myoblast fused each other and formed myofibers in the face of rats, nerve fibers and vessels regeneration could also be found in some samples. Postoperative electromiographs showed the myofibers were active when stimulating the nerve trunk that innervates the engineered facial muscle.
CONCLUSIONTissue engineered method is hopeful to be used as a new technique to reconstruct defects of facial muscle.
