The high expression of HPV31 and 52 L2 fusion protein in E.coli and detection of its immunogenicity
10.3760/cma.j.issn.1003-9279.2013.02.008
- VernacularTitle:HPV31和52 L2融合蛋白的原核高效表达及免疫效果评价
- Keywords:
Papillomavirus,human;
Vaccines;
Expressed sequence tags;
Neutralization tests
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(2):105-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express HPV31 and 52 L2 fusion protein and detect its immunogenicity.Methods According to the amino acid sequences of HPV31 and 52 L2 11-200AA published in the GenBank database,weartificially synthesized the HPV31 and 52 L2 fusion gene which was optimized according to Escherichia coli codon usage and encodes 11-200 amino acid of HPV31 and HPV52 L2,then cloned it into pET-9a vector.The HPV31 and 52 L2 fusion protein was expressed in Prokaryotic expression system and the mice were immunized with the fusion protein after purification.The immunogenicity was characterized in vaccinated mice.Results HPV31 and 52 L2 fusion protein was highly expressed in E.coli,the amount of fusion protein is nearly 20％ of the total bacterial protein.The purified fusion protein with aluminum adjuvant could induce specific high titer of IgG antibodies detected by ELISA,and also induce the neutralizing antibodies against pseudovirus of HPV31 and HPV52 and cross-neutralizing antibodies against pseudovirus of HPV45,58,16,18.Conclusion HPV31 and 52 L2 fusion protein could induce neutralizing and cross-neutralizing antibodies against HPV pseudovirus.It provides laboratory basis for development of HPV L2 protein vaccine.
