Establishment of a purificatory method for alpha-fetoprotein variant by affinity adsorption
10.3760/cma.j.issn.1003-9279.2013.02.015
- VernacularTitle:亲和吸附纯化甲胎蛋白异质体方法的建立
- Keywords:
Alpha-fetoproteins;
Lentils;
Agglutinins
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(2):129-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a purificatory method of alpha-fetoprotein variant (AFP-L3) based on microspincolumn with lens culinaris agglutinin(LCA).Methods LCA was isolated by ammonium sulfate precipitation method from lens culinaris.AFP-L3 affinity adsorption microspincolumns which were made from LCA coupled with activated Sepharose 4B were prepared.By adding into the centrifuge column,serum was absorbed and eluted to purify AFP-L3.The results of purified AFP-L3 detection of 10 cases AFP positive sera by electro-chemiluminescence immunoassay were compared with traditional crossed affinity immunoelectrophoresis.Results 8 of 10 cases AFP-L3 concentration were greater than 5 ng/ml in purified sera.Six cases show positive reaction in affinity immune cross electrophoresis experiment.Conclusion Successfully established purification method of AFP-L3 by affinity absorption based on microspincolumn.The method was more conducive to clinical laboratory applications due to its high sensitive and easy operation.
