Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages
10.3760/cma.j.issn.1003-9279.2013.03.001
- VernacularTitle:发热伴血小板减少综合征病毒在巨噬细胞中的亚细胞定位
- Keywords:
Bunyaviridae;
Macrophages;
Nuclear proteins;
Golgi apparatus;
Endoplasmic reticulum
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.Methods Using two types of human macrophage cell lines THP-1 and U937,the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.Results SFTSV infected macrophage cell lines THP-1 and U937.Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.Conclusion The results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
