The epidemiological characteristics of group C rotavirus in Lulong area and the analysis of diversity of VP6 gene
10.3760/cma.j.issn.1003-9279.2013.03.002
- VernacularTitle:河北卢龙地区猪C组轮状病毒流行状况和VP6基因多样性分析
- Keywords:
Rotavirus;
Epidemiology;
Genes
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(3):164-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the epdimiology characteristics and the diversity of VP6 gene of GCRV in Lulong,and to provide the basis for GCRV in-depth research.Methods 793 stool specimens from porcine with diarrhea or not from Lulong in 2007 and 2008.GCRV was detected by nested multiple reverse transcription-polymerase chain reaction (nested RT-PCR),and analyzed the identity and conducted phylogenetic tree by the seqences.Results The positive rate of GCRV was 16.65％.Porcine GCRV strains of Lulong had significant homology differences.Phylogenetic analysis indicated porcine GCRVs were with significant diversity.Amino acid analysis showed GCRV strains with the same host shared the nearest kinship.Conclusion The infection rate of GCRV was high from 2007 to 2008 in Lulong.Homology and phylogenetic analysis showed that VP6 gene diversity was widespread.The experimental data provided basis for molecular characteristics of porcine GCRVs.
