Full sequence analysis and characterization of the Shenzhen Norovirus strain SZ2010422
10.3760/cma.j.issn.1003-9279.2013.03.007
- VernacularTitle:诺如病毒深圳株SZ2010422全基因组序列测定及分析
- Keywords:
Noroviruses;
Genes;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(3):181-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain information on viral molecular structural and evolutionary characteristics,we conducted the SZ2010422 full-length genomic analysis.Methods Primers were designed by New Orleans full sequence,SZ2010422 full genome was amplified by RT-PCR,the whole genome sequence and the capsid domain amino acid sites was analysised after cloned and sequenced.Results The genome of G lⅡ-4 Norovirus SZ2010422 strain was consist of 7559 bp,it revealed three ORFs composites of the whole genome,ORF1 (5100 bp),ORF2 (1623 bp),ORF3 (807 bp) respectively,ORF1 and ORF2 had 19 nucleotide overlap.By evolutionary comparative analysis found SZ2010422 genomic nucleotide sequences with reference strains of G Ⅱ-4 New Orleansl805 strains the highest homology with a total length of homology was 99.3％,of ORF1 (99.5％),ORF2 (99.2％),ORF3 (98.6％).Phylogenetic analyses showed SZ2010422 belonging to G Ⅱ-4 New Orleans variant.Date of 541 amino acid analyses showed:New Orleans variant strains of popular sites:aa310N or K,→S aa341D→of N,aa359T→S,aa396H→P,aa460H→Y.Conclusion Norovirus SZ2010422 belonged to the G Ⅱ-4 New Orleans variant.In This study,SZ2010422 full sequence can be used not only as a full-length NoV variant sequence standard for future comparison studies,but also as useful material for the public health field by enabling the diagnosis,vaccine development,and prediction of new emerging variants.
