A double antibody sandwich ELISA based assay for titration of severe fever with thrombocytopenia syndrome virus
10.3760/cma.j.issn.1003-9279.2013.03.019
- VernacularTitle:建立基于核蛋白双抗体夹心ELISA的发热伴血小板减少综合征病毒滴定方法
- Keywords:
Bunyaviridae;
Enzyme-linked immunosorbent assay;
Fluorescent antibody technique;
Plaque assay
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(3):215-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an assay for titration of severe fever with thrombocytopenia syndrome virus (SFTSV) based on double antibody sandwich ELISA.Methods A double antibody sandwich ELISA was developed for detection of SFTSV based on SFTSV nucleocapsid (N) protein specific poly-and monoclonal antibodies,procedures were optimized and evaluated.This ELISA based titration assay was compared with fluorescence assasy and plaque assay based titration method.Results The results suggested that the titers obtained by ELISA based method are consistent with those obtained by IFA based method(R =0.999) and the plaque assay titration method (R =0.949).Conclusion The novel ELISA based titration method with high sensitivity and specificity is easy to manage and perform,and can overcome the subjectivity associated with result determination of the fluorescence assay and plaque assay based methods.The novel ELISA based titration method can also be applied to high throughput detection.
