Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression.
- Author:
Ying WANG
1
;
Jianyong FAN
;
Huilan YANG
;
Jianyun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; Cloning, Molecular; Gene Expression Profiling; Herpesvirus 2, Human; genetics; metabolism; MicroRNAs; Oligonucleotide Array Sequence Analysis; Vero Cells; Viral Proteins; genetics; metabolism
- From: Journal of Southern Medical University 2012;32(10):1440-1444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.
METHODSThe full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ- AcGFP1-C1 vector, and the recombinant retrovirus expressing HSV-2 LAT was packaged. Using a microRNA microarray, the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.
RESULTSIn Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression, 5 microRNAs (hsa-miR-23a*, kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5 (hsa-miR-181a-2*, hsa-miR-450b-5p, hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.
CONCLUSIONThe expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.
