Arginase Level in Apheresis Leukocyte-Reduced Platelets Stored for Different Time.

Hao-Bo HUANG; Li-Ping FAN; Shi-Jin WEI; Dan-Hui FU; Feng ZENG; Qing-Hua HUANG

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Arginase Level in Apheresis Leukocyte-Reduced Platelets Stored for Different Time.

Author: Hao-Bo HUANG ¹ ; Li-Ping FAN ¹ ; Shi-Jin WEI ¹ ; Dan-Hui FU ^{2
,

3} ; Feng ZENG ¹ ; Qing-Hua HUANG ¹
Author Information

1. Department of Blood Transfusion, The Union Hospital, Fujian Medical University,Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.
2. Department of Blood Transfusion, Department of Hematology, The Union Hospital, Fujian Medical University,Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China. E-mail: fudanhui@
3. cn.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(6):1879-1882
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the effect of storage time on arginase level and possible source of arginase in apheresis leukocyte-reduced platelets(ALR-Plt).
METHODSThe arginase level and myeloperoxidase(MPO) levels in ALR-Plt and control plasma were detected by ELISA. The relationship between arginase level and MPO level in ALR-Plt was analyzed by correlation analysis.
RESULTSThere was no significant difference of arginase level between ALR-Plt stored less than 3 days and control plasma. However, arginase level in ALR-Plt stored over 3 days was significantly higher than that in ALR-Plt stored less than 3 days and control plasma(P<0.05). There was no significant difference of MPO level in ALR-Plt stored for different times, but the MPO level in ALR-Plt stored for different time was lower than that in control plasma. Correlation analysis showed that arginase level positively correlated with MPO level in ALR-Plt of different storage time (r=0.58).
CONCLUSIONThe arginase level in ALR-Plt stored over 3 days increase significantly. The main possible source of arginase in ALR-Plt is the residual white blood cells, especially neutrophils.