MicroRNA-20a Promotes Osteogenic Differentiation of C3H/10T1/2 Cells through Regulating CKIP-1 Expression.
- VernacularTitle:MicroRNA-20a通过调节CKIP-1促进小鼠C3H/10T1/2成骨分化
- Author:
Jun-Fen CHEN
1
;
Yan-Mei YANG
1
;
Xi-Xi DONG
1
;
Xiao-Hui DONG
2
;
Yu-Han WANG
2
;
Xiao-Hui HUANG
2
;
Xiao-Xia JIANG
3
,
4
;
Jun-Kai CAO
5
Author Information
- Publication Type:Journal Article
- From: Journal of Experimental Hematology 2017;25(1):214-220
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of microRNA-20a(MiR-20a) on osteogenic differentiation of mouse C3H/10T1/2 cells and its regulatory mechanism.
METHODSOsteogenic differentiation of C3H/10T1/2 cells were identified by ALP staining and qRT-PCR. MiR-20a mimics and CKIP-1 siRNA were transfected into C3H/10T1/2 cells respectively with lipo3000. The expression of osteoblast marker genes, miR-20a and CKIP-1 were quantitatively assessed by qRT-PCR.
RESULTSmiR-20a expression was up-regulated during osteoblast differentiation of C3H/10T1/2 cells. Overexpression of miR-20a promoted osteogenic differentiation. Furthermore, miR-20a inhibited the expression of bone formation negative regulator CKIP-1. Additionally, CKIP-1 knockdown promoted osteogenic differentiation.
CONCLUSIONMiR-20a promotes osteogenic differentiation of C3H/10T1/2 cells possibly through inhibiting the expression of CKIP-1.
