Establishment and Application of a Method for Determination of Glycosyltransferase Activity.
- VernacularTitle:糖基转移酶活性测定方法的建立及应用
- Author:
Yan-Lian LIANG
1
,
2
;
Yu-Qing SU
3
;
Yin-Ze ZHANG
3
;
Fan WU
3
;
Da-Cheng LI
3
Author Information
- Publication Type:Journal Article
- From: Journal of Experimental Hematology 2017;25(1):231-234
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determination of glycosyltransferase and to explore the enzyme A, B glycosyltransferase activity in human serum so as to lay the foundation for the determination of enzyme level and enzyme activity.
METHODSThe glycosyltransferase activity kit was used to draw phosphate standard curves in our laboratory. The A and B glycosyltransferase activity were determined by the standard curves.
RESULTSThe standard curves (y=2671.3x-0.596 R=0.9998) for determing glycosyltransferase activity suitable for use in our laboratory were drawn. At the same time the method was set up for determination of A, B glycosyltransferase in human serum.
CONCLUSIONThe establised method of the determination of glycosyltransferase is suitable for common type of enzyme activity and suitable for the A, B glycosyltransferase in human serum.
