Effect of Qingkailing injection on rat CYP1A2 and CYP2D6 activity.
- Author:
Yan LIU
1
,
2
;
Jing WANG
;
Yan-Yan BAO
;
Wei Jiang YU
;
Da-Ling ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caffeine; blood; metabolism; Chromatography, High Pressure Liquid; methods; Cytochrome P-450 CYP1A2; metabolism; Cytochrome P-450 CYP2D6; metabolism; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Injections; Male; Microsomes, Liver; drug effects; enzymology; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2007;32(19):2044-2047
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present study investigates the influence of Qingkailing injection on rat liver CYP1A2 and CYP2D6 activity in vivo and in vitro, respectively.
METHODWe employed HPLC to measure the metabolites of caffeine in the whole blood and calculated the ratio be between the metabolite and caffeine, which was used as index to evaluate the effect of Qingkailing injection on rat CYP1A2 activity in vivo; We also detected the CYP1A2 and CYP2D6 activity in microsomal reconstituted system by analysis of phenacetin metabolism and dextromethorphan metabolism with HPLC.
RESULTThe metabolism of caffeine in treated groups was (15.9 +/- 3.8)%, (14.5 +/- 1.8)%, (12.3 +/- 1.2)%, with different concentration of Qingkailing injection (0.15, 0.3, 0.6 mL x kg(-1)) compared with (16.8 +/- 5.9)% in the control group, which was no significant difference among groups. In rat liver microsomal reconstituted system, Qingkailing injection has no inhibitory effect on CYP2D6 activity while the group with high dose has inhibitory effect on rat CYP1A2.
CONCLUSIONQingkailing injection has no inhibitory effect on rat CYP1A2 and CYP2D6 in vivo and in vitro.
