Analysis of different forms Linderae Radix based on HPLC and NIRS fingerprints.

Wei-Feng DU; Xian-Ke YUE; Yao WU; Wei-Hong GE; Tu-Lin LU; Zhi-Min WANG

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Analysis of different forms Linderae Radix based on HPLC and NIRS fingerprints.

Author: Wei-Feng DU ¹ ; Xian-Ke YUE ¹ ; Yao WU ¹ ; Wei-Hong GE ¹ ; Tu-Lin LU ² ; Zhi-Min WANG ³
Author Information

1. Research Center of Traditonal Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China.
2. College of Pharmaceutical Science, Nanjing University of Traditonal Chinese Medicine, Nanjing 210046, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: HPLC; Linderae Radix; NIRS; cluster analysis; different forms; discriminant analysis
From: China Journal of Chinese Materia Medica 2016;41(19):3551-3556
CountryChina
Language:Chinese
Abstract: Three different forms of Linderae Radix were evaluated by HPLC combined with NIRS fingerprint. The Linderae Radix was divided into three forms, including spindle root, straight root and old root. The HPLC fingerprints were developed, and then cluster analysis was performed using the SPSS software. The near-infrared spectra of Linderae Radix was collected, and then established the discriminant analysis model. The similarity values of the spindle root and straight root all were above 0.990, while the similarity value of the old root was less than 0.850. Two forms of Linderae Radix were obviously divided into three parts by the NIRS model and Cluster analysis. The results of HPLC and FT-NIR analysis showed the quality of Linderae Radix old root was different from the spindle root and straight root. The combined use of the two methods could identify different forms of Linderae Radix quickly and accurately.