Peripheral blood T cell subsets and TH1/TH2 cytokines secretion in children with chronic hepatitis C virus infection.
- Author:
Yi DONG
1
;
Hong-fei ZHANG
;
Hao CHEN
;
Jing LI
;
Xiao-jin YANG
;
Shi-shu ZHU
;
Yun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; CD4 Lymphocyte Count; Child; Cytokines; blood; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Hepatitis C, Chronic; blood; immunology; Humans; Interleukin-10; blood; Interleukin-12; blood; Interleukin-2; blood; Interleukin-4; blood; Leukocytes, Mononuclear; cytology; metabolism; Lymphocyte Count; Male; T-Lymphocyte Subsets; cytology; Th1 Cells; metabolism; Th2 Cells; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the cellular immunological abnormalities in children with chronic hepatitis C virus infection.
METHODS(1) The quantity of the peripheral blood T cell subsets in 16 children with chronic hepatitis C virus infection and 10 healthy blood donors was detected by FACS. (2) The levels of the TH1/TH2 cytokines secretion of PBMC in patients and healthy blood donors were detected by ELISA.
RESULTS(1) Compared with normal controls, there was no significant difference in the percentage of CD4+ cells. The percentage of CD8+ cells was significantly higher than that of controls (P < 0.05). The percentage of CD3+ cells was higher and the ratio of CD4+/CD8+ cells was lower, but the difference was not significant (P > 0.05). (2) Compared with the cytokine level of normal control, the levels of IFN-gamma, IL-10 and TNF-alpha notably increased (P < 0.01) while IL-2, IL-4, and IL-12 were not detected in the culture supernatant of PBMCs from both normal control and patients.
CONCLUSIONThere is an imbalance in peripheral blood T cell subsets and disturbance in cellular immunity in children with chronic hepatitis C virus infection, which may be associated with HCV persistent infection.
