Characteristic of nuclear antigen 1 gene and latent membrane protein 1 gene of Epstein-Barr virus in primary EBV infection in children in Beijing area in 2005-2010
10.3760/cma.j.issn.1003-9279.2012.05.010
- VernacularTitle:2005-2010年北京地区儿童原发性EBV感染流行株EBNA1与LMP1基因特征分析
- Author:
Jun-Hong AI
1
;
Zheng-De XIE
;
Chun-Yan LIU
;
Li-Wei GAO
;
Jing YAN
Author Information
1. 100045,首都医科大学附属北京儿童医院北京市儿科研究所病毒室 儿科学国家重点学科 省部共建儿科重大疾病研究重点实验室
- Keywords:
Epstein-Barr virus infections;
Epstein-Barr virus nuclear antigens;
Membrane proteins;
Child,hospitalized
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(5):352-355
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristic of nuclear antigen 1 gene and latent membrane protein 1 gene of Epstein-Barr virus in primary EBV infection in children in Beijing area in 2005-2012.Methods Polymerase chain reaction (PCR) was used to amplify the EBNA-3C,EBNA1 and LMP1 genes.The amplified products were sequenced directly and the sequences were analyzed by BioEdit 7.0.9 and MEGA 4.0.2.Results Type A EBV was detected in 98％ samples.Nucleotide sequence analysis of the carboxy-terminal region of EBNA1 showed that Vvv1 was deteted in 98％ samples.DNA sequence analysis of LMP1 C-terminus indicated that China 1 was 90％ in this study.There were no significant differences in the frequency of Vvv1 and China 1 between the IM and HLH samples (P =1.00).Linkage analysis of EBV types,EBNA1 and LMP1 variants indicated that 90％ of EBV type A was associated with EBNA1-Vvv1 variant and LMP1-China 1 variant in 40 cases.Full length of LMP1 gene was successfully amplified in 35 cases.Four Chinese groups (CG1-4) were identified.The percentage of CG1-CG4 were 85％,6％,6％ and 3％,respectively.Conclusion EBV type A is predominant in primary EBV infection in children in Beijing Area.EBNA1-Vvv1 and LMP1-China 1 variants were predominant genotypes in this area.There is a high linkage between EBNA1-Vvv1 variant and LMP1-China 1 variant.Four Chinese groups (CG1-4) were identified according to the full length of LMP1 gene and CG1 was the most prevalent.
