Changes of Peripheral Blood Marrow-Derived Suppressor Cell Level after Chemotherapy Induction Remission by VDLP Regimen and Their Relationship with Immune System in B-ALL Children.

Xue-Mei GUO; Yong-Jun FANG; Cheng-Lan LV; Yong-Ren WANG; Xiao-Yan SUN

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Changes of Peripheral Blood Marrow-Derived Suppressor Cell Level after Chemotherapy Induction Remission by VDLP Regimen and Their Relationship with Immune System in B-ALL Children.

VernacularTitle:B-ALL患儿VDLP化疗缓解后髓源抑制性细胞的水平变化及其与免疫系统的关系
Author: Xue-Mei GUO ¹ ; Yong-Jun FANG ^{2
,

3} ; Cheng-Lan LV ⁴ ; Yong-Ren WANG ¹ ; Xiao-Yan SUN ¹
Author Information

1. Department of Hematology and Oncology, The Affiliated Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
2. Department of Hematology and Oncology, The Affiliated Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China. E-mail:fyj322@
3. cn.
4. Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2017;25(6):1611-1614
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the changes of peripheral blood marrow-derived suppressor cell level after chemotherapy induction remission by regimen consisting of vincristine, daunorubicin, L-asparaginase and prednisone (VDLP) and to analyze their relationship with immume system in B-ALL children.
METHODSThirty B-ALL children after induction remission by VDLP regimen from August 2015 to August 2016 were selected as B-ALL group and 30 normal healthy children were selected as control group. The peripheral blood in 2 groups was collected and detected by flow cytometry, then the ratios of CD30cells and CD33HLA-DRmarrow-derived suppressor cells, CD14CD33HLA-DRmarrow-derived suppressor cells and CD15CD33HLA-DRmarrow-derived suppressor cells were calculated, and their changes after induction remission by VDLP regimen and the relationship with immune system were analyzed.
RESULTSAfter treatment the ratio of CD33cells in peripheral blood of B-ALL group and control group was not significantly different (P> 0.05), moreover, the ratio of CD33cells in B-ALL group was significantly higher than that before treatment (P<0.05), while the ratios of CD33HLA-DRmarrow-derived suppressor cells, CD14CD33HLA-DRmarrow-derived suppressor cells and CD15CD33HLA-DRmarrow-derived suppressor cells in B-ALL group were significantly lower than those in control group (all P<0.05), but the ratios of these cells in B-ALL group were higher than those before treatment, and yet there was no statistical significance (P>0.05).
CONCLUSIONThe ratios of marrow-derived suppressor cells in peripheral blood of B-ALL children in complete remission after treatment with VDLP regimen are higher than those before treatment, but are significantly lower than normal value, which may be related with non-complese recovery of immune system in B-ALL children after treatment.