Predictive value of hypo-osmotic swelling test to identify viable non-motile sperm.
- Author:
William M BUCKETT
1
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aniline Compounds; Cell Survival; physiology; Coloring Agents; Eosine Yellowish-(YS); Fluorescent Dyes; Humans; In Vitro Techniques; Male; Osmotic Pressure; Predictive Value of Tests; Semen; cytology; Sperm Motility; physiology; Spermatozoa; physiology; ultrastructure
- From: Asian Journal of Andrology 2003;5(3):209-212
- CountryChina
- Language:English
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Abstract:
AIMTo determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm.
METHODSSemen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined.
RESULTSThe median sperm density was 5.1 million/mL (IQR 4.3 approximately 13.1) and the median motility was 3.0% (IQR 0 approximately 7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59% viability (range 48 approximately 69) despite the poor standard parameters and 47% (range 33 approximately 61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9% reacted overall (range 40 approximately 59) and 41.7% (range 22 approximately 61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7% in the complete asthenozoospermia subgroup.
CONCLUSIONThe HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia.
