Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.
- Author:
Tian-Jie ZHANG
1
,
2
;
ivyandl.li@hotmail.com.
;
Jian-Zhong HAN
;
Hui-Jun LIU
;
Xiao-Hong LIAO
;
Chen LI
;
Zi-Qiang LUO
Author Information
1. Department of Physiology, Central South University, Changsha 410078, China; Department of Physiology, Xiangnan College, Chenzhou 423000, China; Department of Physiology, Changzhi Medical College, Changzhi 046000, China. E-mail: luozq1962@
2. com; ivyandl.li@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Gene Expression;
Interleukin-10;
metabolism;
Lipopolysaccharides;
Macrophages;
metabolism;
Mice;
Peptide Fragments;
metabolism;
RNA, Messenger;
Uteroglobin;
metabolism
- From:
Acta Physiologica Sinica
2013;65(4):363-369
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secretion from macrophages, and this effect is mediated by UGBP.
