Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca(2+)-sensing receptor-evoked extracellular Ca(2+) influx and NO generation in human umbilical vein endothelial cells.
- Author:
Hui ZHAO
1
,
2
;
Xiao LIANG
;
Hua ZHONG
;
Chun-Jun ZHANG
;
Fang HE
Author Information
1. Department of Pathophysiology/Key Laboratory of Education Ministry of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi 832002, China; Department of Lymphoma and Breast Medicine, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China. fangf2002shz@
2. com.
- Publication Type:Journal Article
- MeSH:
Calcium;
physiology;
Calcium Channel Blockers;
pharmacology;
Calcium Channels;
physiology;
Calcium Signaling;
Fluoresceins;
pharmacology;
Human Umbilical Vein Endothelial Cells;
physiology;
Humans;
Nitric Oxide;
biosynthesis;
Nitric Oxide Synthase Type III;
metabolism;
Receptors, Calcium-Sensing;
physiology
- From:
Acta Physiologica Sinica
2013;65(5):553-561
- CountryChina
- Language:Chinese
-
Abstract:
This paper aims to investigate the effect of store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC) on Ca(2+)-sensing receptor (CaR)-induced extracellular Ca(2+) influx and nitric oxide (NO) generation in human umbilical vein endothelial cells (HUVEC). SOC blocker, non-selective cation channel blocker, ROC agonist and ROC blocker were used separately and combined. Intracellular Ca(2+) concentration ([Ca(2+)]i) was measured by Fura-2/AM loading. The activity of endothelial nitric oxide synthase (eNOS) and the production of NO were determined by the DAF-FM diacetate (DAF-FM DA). The results showed that increases of [Ca(2+)]i, eNOS activity and NO generation induced by CaR agonist Spermine were all reduced after single blocking the SOC or ROC, respectively (P < 0.05). ROC agonist can partially abolish the ROC blocker's effect (P < 0.05). The above mentioned effects evoked by CaR agonist Spermine were further reduced when blocking both SOC and ROC than single blocking SOC or ROC in HUVEC (P < 0.05). In conclusion, these results suggest that the SOC and ROC participate in the processes of CaR-evoked extracellular Ca(2+) influx and NO generation by a synergistic manner in HUVEC.
