Gene profiling after knocking-down the expression of NS gene in prostate cancer PC-3 cells.
- Author:
Ran-lu LIU
1
;
Yong XU
;
Zhi-hong ZHANG
;
Meng WANG
;
Jian-tao SUN
;
Yue ZHANG
;
Sheng-zhi LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cyclin D1; metabolism; Cyclin-Dependent Kinase Inhibitor Proteins; metabolism; GTP-Binding Proteins; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Histone Deacetylase 1; metabolism; Humans; Male; Nuclear Proteins; genetics; Oligonucleotide Array Sequence Analysis; Prostatic Neoplasms; genetics; metabolism; pathology; RNA Interference; Signal Transduction
- From: Chinese Journal of Oncology 2009;31(8):561-565
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the genes and possible signal transduction pathways involved in the mechanism of nucleostemin (NS) in the proliferation of prostate cancer.
METHODSOligonucleotide DNA microarray was used to screen the genome changes after knocking-down expression of NS in PC-3 cells and quantitative real-time PCR was used to further confirm the important differentially expressed genes.
RESULTS219 differentially expressed genes were found and theses genes were involved in cell cycle, cell proliferation, signal transduction, cell apoptosis and cell differentiation, etc. INK4 family genes (p15, p16, p18) were up-regulated and cyclin D1, HDAC1 were down-regulated, the main action points were CDK4/6-cyclin D and pRb-E2F1 complexes.
CONCLUSIONNS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. NS is an important G(1)/S checkpoint regulator and its regulatory activity has been certified at gene level.
