Effect of lipopolysaccharide on the stromal cell-derived factor-1 expression in human stem cells from apical papilla
10.3760/cma.j.issn.1002-0098.2015.06.006
- VernacularTitle:脂多糖对人根尖牙乳头干细胞中基质细胞衍生因子1表达的影响
- Author:
Jingyi LIU
1
;
Xiaoying ZOU
;
Xue CHEN
;
Ye CHEN
;
Lin YUE
Author Information
1. 100081,北京大学口腔医学院·口腔医院牙体牙髓科
- Keywords:
Dental papilla;
Stem cells;
Lipopolysaccharides;
Stromal cell-derived factor-1
- From:
Chinese Journal of Stomatology
2015;50(6):346-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of stromal cell-derived factor-1(SDF-1) in human stem cells from apical papilla(SCAP),and to evaluate the effect of lipopolysaccharide(LPS) on SDF-1 expression by SCAP.Methods SCAP were isolated from dental papilla of human immature third molars.The expression of SDF-1 was evaluated by reverse transcription-PCR(RT-PCR).After SCAP being exposed to different concentrations(0.1,1.0,10 mg/L) of LPS for 24 and 48 h,the effect of LPS on cell proliferation and gene expression of SDF-1 was investigated by cell counting kit-8 and real-time PCR respectively,while cells without LPS stimulation were considered as negative control.Results LPS had no significant effect on SCAP proliferation until day 7.RT-PCR assays demonstrated that SCAP expressed SDF-1 mRNA.Different concentrations of LPS significantly promoted the SDF-1 expression in SCAP after 24 h(F=12.102,P=0.002) and 48 h(F=39.054,P＜0.001) exposure,with relative gene expression ratio(experimental/control) increased to 1.4±0.1,2.2±0.4,2.3±0.5 in 24 h group and 2.1±0.4,3.4±0.3,3.8±0.5 in 48 h group.Conclusions Isolated SCAP in cultures have the expression of SDF-1 mRNA.LPS can significantly promote the expression of SDF-1 in SCAP.
