Effect of Lactobacillus acidophilus on metabolizing lactic acid in formula milk: a quantitative analysis of the effect of erythritol
10.3760/cma.j.issn.1002-0098.2015.07.006
- VernacularTitle:赤藓糖醇对嗜酸乳杆菌影响配方奶中乳酸代谢量的定量分析
- Author:
Jun YAO
1
;
Yanjun ZHANG
;
Jiali ZHANG
;
Xiujuan YANG
Author Information
1. 福建医科大学附属口腔医院儿童牙科
- Keywords:
Lactobacillus acidophilus;
Lactic acid;
Chromatography,high pressure liquid;
Erythritol;
Formula milk powder
- From:
Chinese Journal of Stomatology
2015;50(7):408-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the lactic acid productivity of Lactobacillus acidophilus(La) exposed to formula milk containing different concentration of erythritol.Methods La was cultured under anaerobic condition(80％ N2,10％ CO2,10％ H2) at 37 ℃ in five experimental groups(formula milk mixed with different concentrations of erythritol).The five experimental groups contained 1％,2％,4％,6％,and 8％ erythritol,respectively(groups 1％ E-M,2％ E-M,4％ E-M,6％ E-M,8％ E-M).Formula milk served as control group(group M).The lactic acid was analyzed by high performance liquid chromatography(HPLC) at 4 h intervals during 24 h.The peak-area of lactic acid was recorded and used to calculate the concentration of lactic acid through the equation of a standard curve(y=590 244x ± 67 507).ANOVA and Tukey HDS analysis were used to analyze the data.Results The concentration of lactic acid at 24 h was group M [(4.693±0.105) g/L],group 1％E-M[(4.114±0.186) g/L],group 2％ E-M[(3.720±0.158) g/L],group 4％E-M [(3.045 ± 0.152) g/L],group 6％ E-M[(2.971 ± 0.086) g/L],group 8％ E-M[(2.789 ± 0.142) g/L].Statistically significant differences in lactic acid concentrations were found between different time points(P＜0.05) and between different groups(F=187.448,P＜0.05).Moreover,the concentrations of lactic acid in each experimental group was lower than that in control group(P＜0.05).The difference among groups 4％ E-M,6％ E-M,and 8％ E-M were not statistically significant(P＞0.05).Conclusions Erythritol showed the inhibition potential against La in metabolizing lactic acid in formula milk.The effect of erythritol was concentration depended.The higher concentration of erythritol contained in the milk,the better the inhibition potential against La in metabolizing lactic acid.
