Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993.

Author: Quan-xue LAN ¹ ; Qing-hua HU ; Xiao-lu SHI ; Bing WANG ; Yi-man LIN ; Shun-xiang ZHANG ; Jin-quan CHENG ; Biao KAN ; Jian-guo XU
Author Information

1. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Publication Type:Journal Article
MeSH: China; epidemiology; Cholera; epidemiology; microbiology; Electrophoresis, Gel, Pulsed-Field; methods; Humans; Phylogeny; Vibrio cholerae; classification; genetics
From: Chinese Journal of Epidemiology 2007;28(5):491-494
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.
METHODSChromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.
RESULTS39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.
CONCLUSIONThe closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.